Using state-level data from the United States covering the period 1980 to 2020, we explore the effect of right-to-work (RTW) laws on venture capital (VC) investment. Employing a difference-in-differences strategy, we find that the passage of right-to-work laws increases venture capital investment. The results are robust to omitted variable bias, reverse causality and unobservable local economic conditions. We find that the positive effect of RTW laws on VC investments remains significant in states that are highly unionized and technological. • Right to work laws encourage Venture Capital Investment. • Uses state-level data from the United States covering the period 1980 to 2020. • Employs a difference-in-differences strategy. • The passage of right-to-work law increases Venture Capital investment by 80%.