MIS Quarterly 1954

Modifications of Micromanipulative Practice Suitable for Single Cell Isolation and Cultivation of (a) Aerobic and Transiently Chain-forming, (b) Lipophilic and (c) Micro-aerophilic Bacteria

D. Erikson¹; F. M. Masson²

¹ University of Aberdeen · ² Rowett Research Institute, Bucksburn, Aberdeenshire

Abstract

SUMMARY: The methods described allowed successful cultivation of 60 % of single cell isolations in the case of an aerobic, transiently chain-forming and polymorphous organism; 40 % in the case of an organism of related morphology and similar ready viability, but presenting technical difficulties because of the lipophilic nature of the surface membranes; and approximately 2 % in the case of a micro-aerophilic or anaerobic organism of restricted viability.

DOI

10.1099/00221287-11-2-209

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